The Anti-Human CD3 FITC/CD19 APC/CD16 CD56 PE Cocktail is a powerful tool for immunophenotyping studies in humans. This cocktail consists of antibodies labeled with different fluorophores to simultaneously detect multiple cell surface markers, including CD3, CD19, CD16, and CD56. CD3 is a marker found on T cells and is essential for T cell activation and function. CD19 is a B cell marker important for B cell development and activation. CD16 and CD56 are markers commonly found on natural killer (NK) cells, which play a key role in the innate immune response against infected or transformed cells. By using this cocktail, researchers can quickly and accurately identify different immune cell populations in heterogeneous samples, providing valuable insights into immune responses in various diseases such as cancer, infectious diseases, and autoimmune disorders.
This cocktail is ideal for flow cytometry applications and can streamline the analysis of complex immune cell populations in research settings. Overall, the Anti-Human CD3 FITC/CD19 APC/CD16 CD56 PE Cocktail offers a convenient and reliable solution for studying immune cell subsets and their interactions, making it a valuable asset for immunologists and researchers investigating immune-related conditions.
For whole blood samples, add 5 μL Anti-Human CD3-FITC/CD19-APC/CD16+CD56-PE Cocktail to 100 μL anticoagulant-treated blood sample. Mix and incubate the sample at 4°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell with cell staining buffer and discard the supernatant after centrifugation at 300 g for 5 min. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection. For other samples, 1×106 dissociated single cells are centrifuged at 300 g for 5 min with the supernatant discarded. Resuspend the cells with 100 μL cell staining buffer and add 5 μL Anti-Human CD3-FITC/CD19-APC/CD16+CD56-PE Cocktail. Mix and incubate the sample at 4°C in the dark for 30 min. Add cell staining buffer to each tube, centrifuge at 300 g for 5 min and discard the supernatant. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection.
Spectrum:
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
This product is a FCM antibody cocktail made up of FITC Anti-Human CD3 Antibody [Clone: OKT-3] (Mouse IgG2a, κ), APC Anti-Human CD19 Antibody [Clone: CB19] (Mouse IgG1, κ), PE Anti-Human CD16 Antibody [Clone: 3G8] (Mouse IgG1, κ) and PE Anti-Human CD56 Antibody [Clone: 5.1H11] (Mouse IgG1, κ).; CD3 is a heterotetrameric protein consisting of a CD3γ, a CDδ and 2 CD3ε. It forms complex with TCR. OKT-3 recognize human CD3ε. Human CD3 is expressed on the surface of T cells and NKT cells.; CD19 is a single-chain transmembrane glycoprotein expressed on B cells of all stages except plasma cells. It is a common marker for B cells. CD19 is also expressed in follicular dendritic cells. It forms complex with CD21 and CD84, which forms co-receptor with BCR. It takes part in B cell development, activantion and differentiation.; CD16 is a low affinity receptor for the Fc of IgG. Human CD16 has two isoforms, CD16a and CD16b. CD16a is expressed on NK cells, activated monocytes and macrophages. CD16b is expressed on neutrophils. NK cells exert the function of ADCC by binding Fc of IgG through CD16.; CD56 is also call neural cell adhesion molecule (NCAM), expressed on neurons, glia and skeletal muscle cells. In hematopoietic cells, CD56 is also expressed on NK cells and NKT cells. CD56 can be used to detect NK cells, γ/δ T cells and activated CD8+ cells.
