Anti-Human HLA A2,B7 (MHC Class I) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Human HLA-A2/B7 MHC Class I In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the HLA-A2 and B7 proteins, essential components of the major histocompatibility complex (MHC) class I, making it ideal for research in immunology, transplantation, and related fields. With a mouse IgG2a isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays.
Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Enhance your research with this reliable and versatile antibody. HLA-A2 and B7 are glycoproteins found on the surface of almost all nucleated cells, including antigen-presenting cells. They play a critical role in the immune system by presenting peptide antigens to cytotoxic T-cells, thus contributing to the immune response and surveillance against malignancies and infections.
Product Name:
Anti-Human HLA A2,B7 (MHC Class I) In Vivo Antibody - Low Endotoxin
Product Code:
IVMB0130
Size:
1mg, 5mg, 25mg, 50mg, 100mg
Clone:
BB7.6
Protein:
HLA-A2,B7 â‹… MHC Class I
Product Type:
Monoclonal Antibody
Synonyms:
HLA-A, HLA-A2,B7, B.C.HLA-A2
Isotype:
Mouse IgG1
Reactivity:
Human
Immunogen:
Solubilized HLA-B7 antigen
Applications:
FC
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Purity:
≥95% monomer by analytical SEC >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:
FC
Reactivity:
Human
Host Species:
Mouse
Specificity:
Clone BB7.6 recognizes an epitope shared by the human MHC class I molecule HLA-B.
Antigen Distribution:
HLA-B is ubiquitously expressed on nucleated cells.
Immunogen:
Solubilized HLA-B7 antigen
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Purity:
≥95% monomer by analytical SEC >95% by SDS Page
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
HLA-B antibody, clone BB7.6, recognizes a shared epitope (Bw6) of the major histocompatibility complex (MHC) class I molecule human leukocyte antigen (HLA)-B1. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)2,3. The α-chain consists of three domains (α1, α2, and α3)4. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens5-7. β2M noncovalently associates with α3, which is essential for MHC stability. MHC class I plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation8. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling9. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance10. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity11,12.
