Description
Anti-Human HLA-DP (MHC Class II) Monomorphic In Vivo Antibody - Low Endotoxin
Introducing the Anti-Human HLA-DP [MHC Class II, Monomorphic] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications.
This antibody targets the HLA-DP protein, a crucial component of the major histocompatibility complex (MHC) class II, making it ideal for research in immunology and the study of immune response mechanisms. With an ultra-pure formulation and low endotoxin levels (<1.0 EU/mg), it is perfect for applications such as flow cytometry, immunoprecipitation, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline to ensure stability and efficacy, making it a reliable and versatile tool for enhancing your research. HLA-DP is part of the MHC class II complex, found on the surface of antigen-presenting cells including B cells, dendritic cells, and macrophages. It plays a significant role in the immune system by presenting peptides derived from extracellular proteins to T-helper cells, initiating the immune response. With the Anti-Human HLA-DP antibody from Assay Genie, researchers can delve deeper into the functions and mechanisms of the immune system with precision and confidence.
| Product Name: | Anti-Human HLA-DP (MHC Class II) Monomorphic In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0131 |
| Size: | 1mg, 5mg, 25mg, 50mg |
| Clone: | B7/21 |
| Protein: | HLA-DP Monomorphic |
| Product Type: | Monoclonal Antibody |
| Synonyms: | HLA-DP Monomorphic, HLADP, HLA-DPαβ, DPA and DPB, DPα and DPβ, DPαβ |
| Isotype: | Mouse IgG3 |
| Reactivity: | Human |
| Immunogen: | Unknown |
| Applications: | FC, ICC |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Note: B7/21 is an antibody prone to precipitation at 2-8°C resulting in a slight opaque white liquid. At room temperature liquid is clear and colorless. |
| Applications: | FC, ICC |
| Recommended Usage: | FC |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone B7/21 recognizes a monomorphic epitope on human HLA-DP1, -DP2, -DP3, -DP4, and -DP5. It does not cross-react with HLA-DR or HLA-DQ. |
| Antigen Distribution: | HLA-DP is expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells. |
| Immunogen: | Unknown |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Note: B7/21 is an antibody prone to precipitation at 2-8°C resulting in a slight opaque white liquid. At room temperature liquid is clear and colorless. |
HLA-DP antibody, clone B7/21, recognizes the major histocompatibility complex (MHC) class II molecule Human Leukocyte Antigen - DP isotype (HLA-DP). MHC class II is constitutively expressed on human professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation1. HLA-DP consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain2 encoded by the HLA-DPA1 and HLA-DPB1 genes, respectively, located in the HLA complex of chromosome 6. The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens3. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling4. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation5 and can transduce signals into T cells, enhancing T cell proliferation and activity6. High HLA-DP expression is associated with an increased risk of graft-versus-host disease7. Specific alleles of HLA-DP are associated with autoimmune diseases, including rheumatoid arthritis8.
| Technical Datasheet: | View |
| Protein: | HLA-DP Monomorphic |
| Research Area: | Immunology, Innate Immunity |
