The Anti-Human/Monkey CD154/CD40L Antibody [5C8] -Low Endotoxin Purified is a highly specific monoclonal antibody designed to recognize and bind the CD154 antigen with exceptional affinity and selectivity. This antibody has been developed using advanced hybridoma technology and produced in Mouse hosts, resulting in an Mouse IgG2a, κ antibody that exhibits superior binding characteristics. The product is engineered for use in Human, Monkey samples and has been thoroughly validated across multiple experimental platforms. With a concentration of ≥ 1 mg/mL, this antibody provides optimal performance in both qualitative and quantitative applications. This makes it an invaluable tool for researchers investigating CD154-related biological processes and cellular functions.
This antibody is particularly well-suited for FCM applications. It has been verified to perform reliably with Verified Samples in FCM: HEK293T cells transfected with pcDNA3.1 plasmid encoding Human CD154 gene. The high purity and specificity of this reagent ensure minimal cross-reactivity and background noise, enabling researchers to obtain clear and reproducible results. Its versatility extends across multiple experimental methodologies, including flow cytometry, immunohistochemistry, Western blotting, and immunoprecipitation assays. The antibody's robust performance characteristics, combined with its ease of use and compatibility with standard laboratory protocols, make it an excellent choice for both basic research and translational studies. Researchers utilizing this antibody can expect consistent, high-quality results that facilitate meaningful scientific discoveries and advance understanding of CD154 function in health and disease.
Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Storage Buffer:
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.