Description
Anti-Human TCR Vα24-Jα18 (iNKT cell) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Human TCR Vα24-Jα18 [iNKT] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the TCR Vα24-Jα18 protein, a key component of invariant natural killer T (iNKT) cells, making it ideal for research in immunology and related fields. With a mouse IgG1 isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody. TCR Vα24-Jα18 is an invariant T cell receptor element specifically expressed on human iNKT cells, a unique subset of T lymphocytes that play a pivotal role in both innate and adaptive immunity. iNKT cells are crucial for rapid cytokine production and immune regulation, participating in responses against infections, tumors, and autoimmune diseases.
| Product Name: | Anti-Human TCR Vα24-Jα18 (iNKT cell) In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0136 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 6B11 |
| Protein: | TCR Vα24-Jα18 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | TCR Vα24-Jα18, TCR Vα24-JαQ, invariant NKT cell, iNKT |
| Isotype: | Mouse IgG1 κ |
| Reactivity: | Human |
| Applications: | CyTOF®, FC |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | CyTOF®, FC |
| Recommended Usage: | FC The suggested concentration for this TCR Vα24-Jα18 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone 6B11 recognizes the invariant CDR3 region of the human Vα24-Jα18 TCR. |
| Antigen Distribution: | The Vα24-Jα18 TCR is expressed on iNKT cells. |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
TCR Vα24-Jα18 antibody, 6B11, recognizes the CDR3 region of the V alpha 24 and J alpha 18 TCR alpha locus (Vα24-Jα18). The Vα24-Jα18 semi-invariant TCR is expressed on a highly conserved T cell subset, named invariant natural killer T (iNKT cells), which also express NK cell-associated molecules. The Vα24-Jα18 TCR recognizes lipid antigens, such as α-galactosylceramide (α-GalCer), presented by the MHC class I-like molecule CD1d1. Following activation, iNKT cells rapidly proliferate and produce large amounts of IL-4, IFNγ, and cytolytic mediators2,3. iNKT cells have both inflammatory and immunoregulatory functions and are implicated in anti-tumor immunity, protection against infections, suppression of autoimmunity, and the development of inflammatory diseases4.
| Technical Datasheet: | View |
| Protein: | TCR Vα24-Jα18 |
| Ligand/Receptor: | Glycolipid loaded CD1d |
| Research Area: | Immunology |
| Mouse IgG1 Isotype Control [HKSP] | |
|---|---|
| Clone | HKSP |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Low Endotoxin |
| Mouse IgG1 Isotype Control [HKSP84] | |
|---|---|
| Clone | HKSP84 |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Low Endotoxin |
