The Anti-Lactic Acid Histone H3K27 Antibody (CAB18825) is a valuable tool for researchers studying histone modifications related to lactic acid accumulation in cells. This polyclonal antibody, produced in rabbits, has been specifically developed to target the histone H3 lysine 27 residue, which is known to be involved in gene expression regulation.With high reactivity towards human samples, this antibody has been validated for use in techniques such as Western blotting, allowing for precise detection and analysis of histone H3K27 modifications. By specifically binding to the target protein, researchers can investigate the impact of lactic acid accumulation on histone modifications in various cell types, providing insights into potential mechanisms underlying metabolic reprogramming and disease development.
The study of histone modifications in response to lactic acid accumulation is crucial for understanding cellular metabolism, gene regulation, and the development of diseases such as cancer. By utilizing the Anti-Lactic Acid Histone H3K27 Antibody, researchers can advance their research in fields such as metabolism, epigenetics, and cancer biology, ultimately contributing to the discovery of potential therapeutic targets.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Lactic acid-Histone H3-K27 Rabbit pAb (CAB18825) at 1:1000 dilution.C6 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
