The Lactic acid -Histone H4-K5 Antibody (CAB18829) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, is highly specific and sensitive for detecting the histone H4 protein with lysine 5 acetylation. Validated for use in various experimental applications, including Western blot and immunohistochemistry, this antibody allows for accurate detection and visualization of histone H4 (K5) in human samples.Histone modifications, such as acetylation of lysine residues, play a crucial role in the regulation of gene expression and chromatin structure. The acetylation of histone H4 at lysine 5 is associated with active transcription and is linked to processes such as cell proliferation, differentiation, and DNA repair.
This antibody is validated for use in WB, ELISA, DB applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Lactic acid -Histone H4-K5 Antibody
SKU:
CAB18829
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
GRGK G
Tested Applications:
WBELISADB
Recommended Dilution:
WB
1:500 - 1:2000
DB
1:500 - 1:4000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method
Affinity purification
Gene ID
8359
RRID
AB_2862464
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from MCF7 cells, using Lactic acid -Histone H4-K5 Rabbit pAb (CAB18829) at 1:1000 dilution. MCF7 cells were treated with Sodium lactate(100mM) for 24h. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Western blot analysis of lysates from NIH/3T3 cells, using Lactic acid -Histone H4-K5 Rabbit pAb (CAB18829) at 1:1000 dilution. NIH/3T3 cells were treated with Sodium lactate(100mM) for 24h. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Dot-blot analysis of all sorts of peptides using Lactic acid -Histone H4-K5 antibody (CAB18829) at 1:4000 dilution.
