The LCAT Monoclonal Antibody (CAB19228) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, demonstrates high reactivity with human samples and is validated for use in various applications including Western blot and immunofluorescence.LCAT, or Lecithin-Cholesterol Acyltransferase, plays a vital role in the reverse cholesterol transport pathway by catalyzing the esterification of cholesterol in lipoproteins. Dysregulation of LCAT activity has been linked to dyslipidemia, atherosclerosis, and cardiovascular diseases, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
LCAT Monoclonal Antibody
SKU:
CAB19228
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC2383
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LCAT, lecithin-cholesterol acyltransferase
Positive Sample:
Mouse plasma, Rat plasma
Cellular Localization:
Secreted.
Calculated MW:
50kDa
Observed MW:
60kDa
This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency.
Purification Method
Affinity purification
Gene ID
3931
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse plasma, using LCAT Rabbit mAb (CAB19228) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Western blot analysis of lysates from rat plasma, using LCAT Rabbit mAb (CAB19228) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
