LI Cadherin/Cadherin-17 Monoclonal Antibody (CAB5286)
The LI Cadherin/Cadherin-17 Monoclonal Antibody (CAB5286) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants. RRID AB_2863498 Gene ID 1015 Swiss Prot Synonym HPT1; CDH16; HPT-1; LI Cadherin/Cadherin-17
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LI Cadherin/Cadherin-17 Monoclonal Antibody
SKU:
CAB5286
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1989
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISAIF-P
Recommended Dilution:
WB
1:1000 - 1:2000
IF-P
1:100 - 1:1000
IHC-P
1:200 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HPT1, CDH16, HPT-1, LI Cadherin/Cadherin-17
Positive Sample:
HT-29 cells, Mouse large intestine
Cellular Localization:
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
92kDa
Observed MW:
120kDa
This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants. RRID AB_2863498 Gene ID 1015 Swiss Prot Synonym HPT1; CDH16; HPT-1; LI Cadherin/Cadherin-17
Purification Method:
Affinity purification
Gene ID:
1015
RRID:
AB_2863498
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using LI Cadherin/Cadherin-17 Rabbit mAb (CAB5286) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using LI Cadherin/Cadherin-17 Rabbit mAb (CAB5286) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human stomach using LI Cadherin/Cadherin-17 Rabbit mAb (CAB5286) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of Human colon cancer using LI Cadherin/Cadherin-17 Rabbit mAb (CAB5286,dilution 1:100)(Red). DAPI was used for nuclear staining (blue). Objective: 40x.
Immunofluorescence analysis of paraffin-embedded rat rectum using LI Cadherin/Cadherin-17 Rabbit mAb (CAB5286) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
