The LIS1 Monoclonal Antibody (CAB3696) is a high-quality antibody developed for reliable detection and analysis of target proteins. This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LIS1 Monoclonal Antibody
SKU:
CAB3696
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2075
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:50 - 1:200
IP
0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MDS, LIS1, LIS2, MDCR, NudF, PAFAH
Positive Sample:
U-87MG, Mouse testis, Mouse brain
Cellular Localization:
Axon Cytoplasm, Cell Cortex, Central Region Of Growth Cone, Centrosome, Cytoplasmic Microtubule, Cytosol, Extracellular Exosome, Glutamatergic Synapse, Neuron Projection, Nuclear Envelope, Perinuclear Region Of Cytoplasm, Schaffer Collateral - Ca1 Synapse.
Calculated MW:
47kDa
Observed MW:
47kDa
This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum.
Purification Method
Affinity purification
Gene ID
5048
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using LIS1 Rabbit mAb (CAB3696) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Immunofluorescence analysis of C6 cells using LIS1 Rabbit mAb (CAB3696) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using LIS1 Rabbit mAb (CAB3696) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
