The MERS-CoV Spike RBD Antibody (CAB20565) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, specifically targets the receptor-binding domain (RBD) of the MERS-CoV Spike protein, which is critical for viral entry into host cells.The MERS-CoV Spike RBD Antibody is highly reactive with samples from humans, making it an excellent choice for studies on MERS-CoV infection and transmission. It has been validated for use in Western blot applications, enabling the detection and analysis of the MERS-CoV Spike protein in various cell types.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against MERS-CoV samples.
Product Name:
MERS-CoV Spike RBD Antibody
SKU:
CAB20565
Size:
20μL, 100μL
Reactivity:
MERS-CoV
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
293T
Calculated MW:
149kDa
Observed MW:
200kDa
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Purification Method
Affinity purification
Gene ID
14254594
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MERS-CoV Spike RBD Rabbit pAb (CAB20565) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of 293T cells transfected with MERS-CoV Spike(GFP-Tag) protein and untreated 293T cells use MERS-CoV Spike RBD Rabbit pAb (CAB20565) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
