The MGRN1 Antibody (CAB20476) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, displays high reactivity with human samples and has been validated for use in various research applications, including Western blot and immunofluorescence.MGRN1, also known as mahogunin RING finger-1, plays a crucial role in the ubiquitin-proteasome system, which is responsible for degrading damaged or misfolded proteins within cells. Dysregulation of this system has been linked to the development of neurodegenerative disorders, making MGRN1 an important target for research in this area.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
MGRN1 Antibody
SKU:
CAB20476
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Enables ubiquitin-protein transferase activity. Involved in endosome to lysosome transport; negative regulation of signal transduction; and protein monoubiquitination. Located in several cellular components, including early endosome; endoplasmic reticulum; and nucleus.
Purification Method
Affinity purification
Gene ID
23295
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat heart, using MGRN1 Rabbit pAb (CAB20476) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of NIH/3T3 cells using MGRN1 Rabbit pAb (CAB20476) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
