Description
Anti-Mouse 4-1BB In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse 4-1BB In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody crafted for in vivo applications.
This antibody targets the 4-1BB protein (CD137), a crucial costimulatory molecule in T-cell activation and survival, making it an essential tool for research in immunology and related fields. With a rat IgG2a isotype, it ensures remarkable purity and low endotoxin levels (<1.0 EU/mg), thus guaranteeing optimal performance in ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, this antibody is formulated in phosphate-buffered saline to ensure stability and efficacy. Enhance your research endeavors with this reliable and versatile antibody. 4-1BB (CD137) is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on activated T-cells, natural killer cells, dendritic cells, and some tumor cells. It plays a pivotal role in modulating immune responses by promoting T-cell proliferation and survival. By targeting 4-1BB, researchers can delve into the mechanisms of immune regulation and potential therapeutic interventions for cancer and autoimmune diseases.
| Product Name: | Anti-Mouse 4-1BB In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0045 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 3H3 |
| Protein: | 4-1BB |
| Product Type: | Monoclonal Antibody |
| Synonyms: | TNFRSF9, 4-1BB, CD137 Antigen, T-Cell Antigen, ILA, CD137 |
| Isotype: | Rat IgG2a κ |
| Reactivity: | Mouse |
| Immunogen: | Recombinant Mouse CD137 human Fc fusion protein |
| Applications: | ELISA, FA, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | ELISA, FA, WB |
| Recommended Usage: | WB ELISA |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone 3H3 recognizes an epitope on mouse 4-1BB. |
| Antigen Distribution: | 4-1BB is expressed on activated B cells and T cells, macrophages, and dendritic cells. |
| Immunogen: | Recombinant Mouse CD137 human Fc fusion protein |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
4-1BB (CD137) is a 39 kD transmembrane protein that is a member of the tumor necrosis factor (TNF) receptor family and is a co-stimulatory molecule that plays a role in T-cell-mediated proliferative response. When binding its ligand, CD137 provides costimulatory signals to both CD4 and CD8 T cells via the activation of NF-\uf06bB, c-Jun and p38 downstream pathways. Crosslinking of CD137 boosts T cell proliferation, IL-2 secretion, survival and cytolytic activity. Furthermore, it can increase immune activity to eliminate tumors in mice. Agonistic anti-CD137 antibodies have been reported to stimulate a more intense immune system attack on cancers.
| Technical Datasheet: | View |
| Protein: | 4-1BB |
| Ligand/Receptor: | 4-1BB (CDw137) |
| Research Area: | Costimulatory Molecules, Immunology |
