![Anti-Mouse CD11a [I21/7] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/214474/569441/anti-mouse-cd11a-i217-in-vivo-antibody-low-endotoxin__76307.1673496890.jpg?c=2?imbypass=on "Anti-Mouse CD11a [I21/7] In Vivo Antibody - Low Endotoxin")
Description
Anti-Mouse CD11a [I21/7] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD11a [I21/7] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody tailor-made for in vivo applications. This antibody specifically targets the CD11a protein, a crucial molecule involved in leukocyte adhesion and signal transduction, making it indispensable for research in immunology and related fields.
With a rat IgG2a isotype, this antibody ensures high purity and low endotoxin levels (<1.0 EU/mg), making it ideal for ELISA, flow cytometry, immunohistochemistry, and other related assays. Available in various sizes, it is meticulously formulated in phosphate-buffered saline for optimal stability and efficacy. Elevate your research with this dependable and versatile antibody. CD11a is an integral membrane protein that is part of the integrin alpha chain family, expressed on the surface of leukocytes. It plays a pivotal role in the immune system by mediating adhesion of leukocytes to other cell types and facilitating signaling pathways crucial for immune responses.
| Product Name: | Anti-Mouse CD11a [I21/7] In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0139 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | I21/7 |
| Protein: | CD11a |
| Product Type: | Monoclonal Antibody |
| Synonyms: | LFA-1α |
| Isotype: | Rat IgG2a |
| Reactivity: | Mouse |
| Immunogen: | Whole T cells Lymphoma BW5147 cells |
| Applications: | FA, FC |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | FA, FC |
| Recommended Usage: | FC The suggested concentration for this I21/7 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone I21/7 recognizes an epitope on mouse CD11a. |
| Antigen Distribution: | CD11a is present on thymocytes, blood lymphocytes, bone marrow cells and certain lymphoma and macrophage-like cell lines. |
| Immunogen: | Whole T cells Lymphoma BW5147 cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or β may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the β2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/β heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the β2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
| Technical Datasheet: | View |
| Protein: | CD11a |
| Function: | Intercellular adhesion |
| Ligand/Receptor: | ICAM-1 (CD54), ICAM-2(CD102), ICAM-3(CD50) |
| Research Area: | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction |
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