Description
Anti-Mouse CD11b In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD11b In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD11b protein, a crucial molecule in immune cell adhesion and migration, making it ideal for research in immunology and related fields. With a rat IgG2b isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody. CD11b, also known as integrin alpha M, is expressed on the surface of immune cells such as monocytes, macrophages, granulocytes, and natural killer cells. It plays a significant role in the innate immune system by mediating cell adhesion, migration, and phagocytosis through interaction with complement component iC3b and other ligands.
| Product Name: | Anti-Mouse CD11b In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0042 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | M1/70 |
| Protein: | CD11b |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Mac-1, Integrin αm chain, C3biR, CR3, Mo1, ITGAM |
| Isotype: | Rat IgG2b κ |
| Reactivity: | Mouse |
| Immunogen: | B10 mouse spleen cells enriched for T cells |
| Applications: | CyTOF®, FA, FC, ICC, IHC FF, PhenoCycler® |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | CODEX®, CyTOF®, FA, FC, ICC, IHC FF |
| Recommended Usage: | FC The suggested concentration for this M1/70 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone M1/70 recognizes the α subunit (CD11b) of the mouse CD11b/CD18 complex. |
| Antigen Distribution: | CD11b is expressed on human peripheral blood lymphocytes, NK lymphocytes, monocytes, granulocytes, a subset of T-cells and macrophages. |
| Immunogen: | B10 mouse spleen cells enriched for T cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or β may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the β2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/β heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the β2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
| Technical Datasheet: | View |
| Protein: | CD11b |
| Function: | Adhesion, chemotaxis |
| Ligand/Receptor: | ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen |
| Research Area: | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers |
