![Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Ultra Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/214479/569389/anti-mouse-cd120a-tnfr1-55r-170-in-vivo-antibody-ultra-low-endotoxin__51795.1770916893.jpg?c=2?imbypass=on "Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Ultra Low Endotoxin")
Description
Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse CD120a - Ultra Low Endotoxin In Vivo Antibody from Assay Genie, a highly specific monoclonal antibody designed for in vivo research applications. This antibody targets CD120a (TNFR1/55R), a key receptor in the TNF-α signaling pathway, making it an exceptional tool for studies in inflammation, apoptosis, and immune response. Classified with mouse IgG isotype, it ensures exceptional purity and ultra-low endotoxin levels (<1.0 EU/mg), thereby meeting rigorous experimental standards and reducing the risk of endotoxin-related interference. This antibody is ideal for applications such as in vivo functional assays, flow cytometry, immunohistochemistry, and other critical research protocols. Available in multiple sizes to accommodate diverse research needs, it is formulated in phosphate-buffered saline for maximum stability and effectiveness.
Enhance the precision and reliability of your research with this robust antibody from Assay Genie. CD120a, also known as TNFR1 (tumor necrosis factor receptor 1), is a receptor found on the surface of a variety of cell types that binds to tumor necrosis factor-alpha (TNF-α). This interaction is crucial for mediating inflammatory and apoptotic pathways. The receptor is implicated in numerous pathological conditions including autoimmune diseases, making it an important target for therapeutic research and intervention.
| Product Name: | Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0144 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 55R-170 |
| Protein: | TNFR1 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | TNFR-I, TNFRSF1A, P55, P60 |
| Isotype: | Armenian Hamster IgG |
| Reactivity: | Mouse |
| Immunogen: | Purified Recombinant Mouse TNFR1 (>98%) |
| Applications: | B, FC, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, FC, IP, WB |
| Recommended Usage: | eLISA This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml. WB To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents and a chromogenic substrate, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions. FC |
| Reactivity: | Mouse |
| Host Species: | Armenian Hamster |
| Specificity: | Clone 55R-170 recognizes mouse CD120a. |
| Antigen Distribution: | CD120a is constitutively expressed in most tissues. |
| Immunogen: | Purified Recombinant Mouse TNFR1 (>98%) |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
Tumor Necrosis Factor Receptor Type I (TNFRI) is a 55kD Type I transmembrane protein. TNFRI is expressed independently of TNFRII at low levels on a wide variety of cell types. TNFRI binds TNF-α and TNF-β (also known as LT-α). The subsequent signaling is known to be important for inducing cytolytic activity, antiviral activity, expression of manganous superoxide dismutase and intercellular adhesion molecule (ICAM), IL-6 mRNA accumulation and NF-ĸB induction. TNFRI carries an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF Receptor I associated death domain protein). Binding of 55R-170 antibody to TNFRI has been shown to block in vitro and in vivo receptor signaling initiated by ligand binding.
| Technical Datasheet: | View |
| Protein: | TNFR1 |
| Function: | Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation |
| Ligand/Receptor: | TNF-α, LT-α (TNF-β) |
| Research Area: | Immunology, Innate Immunity |
| Armenian Hamster IgG Isotype Control | |
|---|---|
| Clone | PIP |
| Isotype | Armenian Hamster IgG1 |
| Endotoxin Level | Ultra Low Endotoxin |
![Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Ultra Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/608x608/products/214479/569389/anti-mouse-cd120a-tnfr1-55r-170-in-vivo-antibody-ultra-low-endotoxin__51795.1770916893.jpg?c=2 "Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Ultra Low Endotoxin")
![Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214477/569494/anti-mouse-cd120a-tnfr1-55r-170-in-vivo-antibody-low-endotoxin__20332.1770914665.jpg?c=2 "Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Low Endotoxin")
![Anti-Mouse CD120a (TNFR1) [55R-593] In Vivo Antibody - Ultra Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214480/569361/anti-mouse-cd120a-tnfr1-55r-593-in-vivo-antibody-ultra-low-endotoxin__45317.1770916748.jpg?c=2 "Anti-Mouse CD120a (TNFR1) [55R-593] In Vivo Antibody - Ultra Low Endotoxin")
![Anti-Mouse CD120a (TNFR1) [55R-593] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214478/569393/anti-mouse-cd120a-tnfr1-55r-593-in-vivo-antibody-low-endotoxin__98959.1770914568.jpg?c=2 "Anti-Mouse CD120a (TNFR1) [55R-593] In Vivo Antibody - Low Endotoxin")
