The Anti-Mouse CD16/32 Antibody [2.4G2] -Low Endotoxin Purified is a highly specific monoclonal antibody designed to recognize and bind the CD16/32 antigen with exceptional affinity and selectivity. This antibody has been developed using advanced hybridoma technology and produced in Rat hosts, resulting in an Rat IgG2b, κ antibody that exhibits superior binding characteristics. The product is engineered for use in Mouse samples and has been thoroughly validated across multiple experimental platforms. With a concentration of ≥ 1 mg/mL, this antibody provides optimal performance in both qualitative and quantitative applications. This makes it an invaluable tool for researchers investigating CD16/32-related biological processes and cellular functions.
This antibody is particularly well-suited for FCM applications. It has been verified to perform reliably with Verified Samples in FCM: C57/BL6 Mouse splenocytes. The high purity and specificity of this reagent ensure minimal cross-reactivity and background noise, enabling researchers to obtain clear and reproducible results. Its versatility extends across multiple experimental methodologies, including flow cytometry, immunohistochemistry, Western blotting, and immunoprecipitation assays. The antibody's robust performance characteristics, combined with its ease of use and compatibility with standard laboratory protocols, make it an excellent choice for both basic research and translational studies. Researchers utilizing this antibody can expect consistent, high-quality results that facilitate meaningful scientific discoveries and advance understanding of CD16/32 function in health and disease.
CD16a/b, CD32, CD32A/B, FCG2A, FCGR2A/BFCGR3, FCGR3A/B, Fc fragment of IgG low affinity IIIa/b receptor, Fc fragment of IgG low affinity IIIb receptor, Fc fragment of IgG low affinity IIa/b receptor, Fc gamma RIIa/b , Fc gamma receptor III A/B, FcGR
Reactivity:
Mouse
Immunogen:
Recombinant Mouse CD16/32 protein
Applications:
FCM
Clonality:
Monoclonal
Clone No:
2.4G2
Host:
Rat
Isotype:
Rat IgG2b, κ
Concentration:
≥ 1 mg/mL
Target:
CD16/32
Recommended Dilution:
FCM 2 µg/mL(0.5×10⁶-1×10⁶ cells)
Purification:
>98%, Protein A/G purified
Swissprot:
P08508P08101
Storage:
Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Storage Buffer:
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.