Description
Anti-Mouse CD32/CD16 In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse CD32/CD16 In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody meticulously designed for in vivo applications. This antibody targets the CD32 and CD16 proteins, which are vital components of the immune response, making it an indispensable tool for research in immunology and related fields. With an IgG2b isotype, it ensures superior purity and extremely low endotoxin levels (<0.1 EU/mg), ideal for ELISA, flow cytometry, immunohistochemistry, and various other assays. Available in numerous sizes and formulated in phosphate-buffered saline, it guarantees stability and efficacy. Elevate your research with this dependable and versatile antibody.
CD32 (FcγRII) and CD16 (FcγRIII) are receptors expressed on the surface of a range of immune cells, including natural killer (NK) cells, macrophages, neutrophils, and some T-cell subsets. CD32 plays a crucial role in modulating immune responses by binding to the Fc region of immunoglobulins, leading to either activation or inhibition of immune cells. CD16, a low-affinity receptor for the Fc portion of IgG, is essential for antibody-dependent cellular cytotoxicity (ADCC) and other immune functions. Leveraging the Anti-Mouse CD32/CD16 In Vivo Antibody can significantly advance your understanding of immune mechanisms and therapeutic interventions.
| Product Name: | Anti-Mouse CD32/CD16 In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0028 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 2.4G2 |
| Protein: | CD32/CD16 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Fcγ R III/II, Ly-17 |
| Isotype: | Rat IgG2b |
| Reactivity: | Mouse |
| Immunogen: | Sorted pre-B cells |
| Applications: | FC, IHC FF, IP, PhenoCycler®, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | CODEX®, FC, IHC FF, IP, WB |
| Recommended Usage: | FC The suggested concentration for this 2.4G2 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this 2.4G2 antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone 2.4G2 recognizes the FcγIII and FcγII receptors. |
| Antigen Distribution: | These receptors are present on B cells, monocyte/macrophages, NK cells, neutrophils, mast cells and dendritic cells. |
| Immunogen: | Sorted pre-B cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD16 is expressed in two forms: CD16a and CD16b. CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (FcγRIIIB) is a 48 kD GPI-anchored protein whose extracellular domain is over 95% homologous to that of CD16a. CD16 regulates both phagocytosis and antibody-dependent cell-mediated cytotoxicity. It has been reported that CD16 is involved in Natural Killer Cell activation and plays a role in signal transduction. The receptors, CD32 (FcγRIII) and CD16 (FcγRII), are 40-60 kD and bind antibody-antigen immune complexes and mediate adaptive immune responses.
| Technical Datasheet: | View |
| Protein: | CD32/CD16 |
| Function: | Low affinity receptors for IgG |
| Ligand/Receptor: | IgG |
| Research Area: | Immunology, Innate Immunity |
| Rat IgG2b Isotype Control | |
|---|---|
| Clone | 1-2 |
| Isotype | Rat IgG2b kappa |
| Endotoxin Level | Ultra Low Endotoxin |
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