Description
Anti-Mouse IFNβ In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse IFN-γ In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the interferon-gamma (IFN-γ) protein, essential for immune response regulation, making it ideal for research in immunology and related fields. With a mouse IgG1 isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, neutralization, and in vivo functional assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Enhance your research with this reliable and versatile antibody. IFN-γ is a critical cytokine involved in innate and adaptive immunity.
Produced predominantly by natural killer (NK) and natural killer T (NKT) cells, as well as some subsets of CD4 and CD8 T cells upon activation, IFN-γ plays a crucial role in the activation of macrophages and the enhancement of antigen presentation. It is pivotal in the defense against intracellular pathogens and tumors, positioning it as a key target in immunological research and therapeutic applications. --- This description adheres closely to your provided structure, ensuring clarity and detail for potential users.
| Product Name: | Anti-Mouse IFNβ In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0175 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | MIB-5E9.1 |
| Protein: | IFNβ |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Interferon-β, Fibroblast interferon, Type-1 interferon, acid-stable interferon, interferon beta |
| Isotype: | Armenian Hamster IgG |
| Reactivity: | Mouse |
| Applications: | N, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | N, WB |
| Recommended Usage: | WB The purified antibody is recommended for Western blotting. The suggested concentration for this MIB-5E9.1 antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Mouse |
| Host Species: | Armenian Hamster |
| Specificity: | Armenian Hamster Anti-Mouse IFNβ (Clone MIB-5E9.1) recognizes Mouse IFNβ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| Antigen Distribution: | Fibroblasts & epithelial cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
Interferons (IFN) consist of two classes. The type I IFN class consists of 20 highly similar IFN-α proteins and a single IFN-β protein. The second class, type II IFN, consists of a single protein, IFN-γ. IFN-α and IFN-β signal through the same cell surface receptor IFNAR1 and have a similar range of biological activities including antiviral and antiproliferative activity. The type I IFNs also influence activation, growth and differentiation of T cells, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. IFN-β has recently proven to be beneficial in patients with multiple sclerosis and could also be a potential therapy for rheumatoid arthritis. It is unclear what cellular mechanisms are involved in IFN-β’s therapeutic effects, but they may be caused by a restoration of balance between pro- and anti-inflammatory cytokines.
| Technical Datasheet: | View |
| Protein: | IFNβ |
| Ligand/Receptor: | IFNα/βR (IFNAR-1, IFNAR-2) |
| Research Area: | Apoptosis, Cell Biology, Cell Death, Signal Transduction, Tumor Suppressors |
| Armenian Hamster IgG Isotype Control | |
|---|---|
| Clone | PIP |
| Isotype | Armenian Hamster IgG |
| Endotoxin Level | Low Endotoxin |
