![Anti-Mouse PD-L1 (B7-H1) [10F.9G2] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/214430/569309/anti-mouse-pd-l1-b7-h1-10f.9g2-in-vivo-antibody-low-endotoxin__16181.1770914470.jpg?c=2?imbypass=on "Anti-Mouse PD-L1 (B7-H1) [10F.9G2] In Vivo Antibody - Low Endotoxin")
Description
Anti-Mouse PD-L1 (B7-H1) [10F.9G2] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse PD-L1 [10F.9G2] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the PD-L1 protein, crucial in immune response regulation, making it ideal for research in immunology and cancer studies. With a rat IgG2b isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody. PD-L1, also known as B7-H1, is an immune checkpoint protein expressed on the surface of various cell types, including tumor cells and immune cells. It plays a significant role in the immune system by inhibiting T-cell activation and allowing cancer cells to evade immune detection through interaction with the PD-1 receptor on T-cells.
| Product Name: | Anti-Mouse PD-L1 (B7-H1) [10F.9G2] In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0095 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 10F.9G2 |
| Protein: | PD-L1 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | B7-H1, CD274 |
| Isotype: | Rat IgG2b κ |
| Reactivity: | Mouse |
| Immunogen: | UnKnown |
| Applications: | B, FC, IHC FF, PhenoCycler®, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CODEX®, FC, IHC FF, WB |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone 10F.9G2 recognizes an epitope on mouse PD-L1. |
| Antigen Distribution: | PD-L1 is present on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes.. |
| Immunogen: | UnKnown |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
PD-1 is a 50-55 kD member of the B7 Ig superfamily. PD-1 is also a member of the extended CD28/CTLA-4 family of T cell regulators and is suspected to play a role in lymphocyte clonal selection and peripheral tolerance. The ligands of PD-1 are PD-L1 and PD-L2, and are also members of the B7 Ig superfamily. PD-1 and its ligands negatively regulate immune responses. PD-L1, or B7-Homolog 1, is a 40 kD type I transmembrane protein that has been reported to costimulate T cell growth and cytokine production. The interaction of PD-1 with its ligand PD-L1 is critical in the inhibition of T cell responses that include T cell proliferation and cytokine production. PD-L1 has increased expression in several cancers. Inhibition of the interaction between PD-1 and PD-L1 can serve as an immune checkpoint blockade by improving T-cell responses In vitro and mediating preclinical antitumor activity. Within the field of checkpoint inhibition, combination therapy using anti-PD1 in conjunction with anti-CTLA4 has significant therapeutic potential for tumor treatments. PD-L2 is a 25 kD type I transmembrane ligand of PD-1. Via PD-1, PD-L2 can serve as a coinhibitor of T cell functions. Regulation of T cell responses, including enhanced T cell proliferation and cytokine production, can result from mAbs that block the PD-L2 and PD-1 interaction.
| Technical Datasheet: | View |
| Protein: | PD-L1 |
| Ligand/Receptor: | PD-1 (PDCD1) |
| Research Area: | Cancer, Costimulatory Molecules, Immunology |
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