![Anti-Mouse PD-L1 (B7-H1) [10F.9G2] In Vivo Antibody - Ultra Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/214431/569423/anti-mouse-pd-l1-b7-h1-10f.9g2-in-vivo-antibody-ultra-low-endotoxin__99501.1770916458.jpg?c=2?imbypass=on "Anti-Mouse PD-L1 (B7-H1) [10F.9G2] In Vivo Antibody - Ultra Low Endotoxin")
Description
Anti-Mouse PD-L1 (B7-H1) [10F.9G2] In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse PD-L1 [B7-H1] [10F.9G2] In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody effectively targets the PD-L1 (B7-H1) protein, an immune checkpoint molecule that plays a critical role in tumor immunology and immune tolerance, making it an essential tool for research in oncology, immunotherapy, and related fields. With a rat IgG2b kappa isotype, it ensures superior purity and ultra-low endotoxin levels (<0.01 EU/μg), ideal for avoiding immune response biases in sensitive in vivo assays. The antibody is formulated in phosphate-buffered saline without preservatives to maintain stability and effectiveness.
PD-L1 (Programmed Death-Ligand 1) is a transmembrane protein expressed on both tumor cells and some immune cells. It interacts with the PD-1 receptor on T-cells to inhibit T-cell activation and proliferation, thus contributing to immune escape mechanisms employed by tumors. By targeting PD-L1, this antibody can be used to investigate and modulate immune checkpoint pathways, offering significant insights into cancer biology and therapeutic development. Enhance your research with the reliable and versatile Anti-Mouse PD-L1 [B7-H1] [10F.9G2] In Vivo Antibody, available in multiple sizes to suit your experimental needs.
| Product Name: | Anti-Mouse PD-L1 (B7-H1) [10F.9G2] In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0096 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 10F.9G2 |
| Protein: | PD-L1 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Programmed Death Ligand 1, B7-H1, PD-L1, CD274 |
| Isotype: | Rat IgG2b κ |
| Reactivity: | Mouse |
| Immunogen: | Mouse CD274 |
| Applications: | B, FA, IHC FF, PhenoCycler®, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CODEX®, FA, IHC FF, WB |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone 10F.9G2 recognizes an epitope on mouse PD-L1. |
| Antigen Distribution: | PD-L1 is present on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. |
| Immunogen: | Mouse CD274 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
PD-1 is a 50-55 kD member of the B7 Ig superfamily. PD-1 is also a member of the extended CD28/CTLA-4 family of T cell regulators and is suspected to play a role in lymphocyte clonal selection and peripheral tolerance. The ligands of PD-1 are PD-L1 and PD-L2, and are also members of the B7 Ig superfamily. PD-1 and its ligands negatively regulate immune responses. PD-L1, or B7-Homolog 1, is a 40 kD type I transmembrane protein that has been reported to costimulate T cell growth and cytokine production. The interaction of PD-1 with its ligand PD-L1 is critical in the inhibition of T cell responses that include T cell proliferation and cytokine production. PD-L1 has increased expression in several cancers. Inhibition of the interaction between PD-1 and PD-L1 can serve as an immune checkpoint blockade by improving T-cell responses In vitro and mediating preclinical antitumor activity. Within the field of checkpoint inhibition, combination therapy using anti-PD1 in conjunction with anti-CTLA4 has significant therapeutic potential for tumor treatments. PD-L2 is a 25 kD type I transmembrane ligand of PD-1. Via PD-1, PD-L2 can serve as a coinhibitor of T cell functions. Regulation of T cell responses, including enhanced T cell proliferation and cytokine production, can result from mAbs that block the PD-L2 and PD-1 interaction.
| Technical Datasheet: | View |
| Protein: | PD-L1 |
| Ligand/Receptor: | PD-1 (PDCD1) |
| Research Area: | Cancer, Costimulatory Molecules, Immunology |
| Rat IgG2b Isotype Control | |
|---|---|
| Clone | 1-2 |
| Isotype | Rat IgG2b kappa |
| Endotoxin Level | Ultra Low Endotoxin |
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