Description
Anti-Mouse/Rat CD126 (IL-6Rα) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse/Rat CD126 (IL-6R) In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications.
This antibody targets the CD126 protein, also known as IL-6 receptor, which is pivotal for the regulation of immune responses and inflammation, making it ideal for research in immunology and related fields. With a mouse IgG2b isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Enhance your research with this reliable and versatile antibody. CD126 (IL-6R) is a part of the interleukin-6 (IL-6) receptor complex, involved in the immune system's response to infection and injury. It plays a major role in inflammatory and immune responses by mediating the effects of IL-6 signaling. Understanding and manipulating this receptor is crucial for studying diseases linked to inflammation and immune regulation, such as autoimmune diseases and cancer.
| Product Name: | Anti-Mouse/Rat CD126 (IL-6Rα) In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0181 |
| Size: | 1mg |
| Clone: | D7715A7 (15A7) |
| Protein: | CD126 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | IL-6 Receptor α chain, IL-6R |
| Isotype: | Rat IgG2b κ |
| Reactivity: | Mouse |
| Immunogen: | OKT4 hybridoma cells |
| Applications: | FA, FC, N, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | FA, FC, N, WB |
| Recommended Usage: | FC The suggested concentration for this D7715A7 (15A7) antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this D7715A7 (15A7) antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone D7715A7 (Clone 15A7) recognizes an epitope on mouse CD126. |
| Antigen Distribution: | CD126 is expressed on activated T and B cells, monocytes, hepatocytes and plasma cells. |
| Immunogen: | OKT4 hybridoma cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD126 (IL-6Rα) is an 80 kD type I cytokine receptor α chain that is a member of the Ig superfamily that can form a heterodimer with IL-6Rβ chain to form the high affinity IL-6 receptor. The IL-6/IL-6R complex is a potent pleiotropic cytokine that mediates cell growth and differentiation and has significant involvement in immune response. Inadequate production of IL-6 and IL-6R are thought to play a part in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer.
| Technical Datasheet: | View |
| Protein: | CD126 |
| Research Area: | Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Signal Transduction |
