Description
Anti-Mouse TNFα In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse TNF In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the TNF (Tumor Necrosis Factor) protein, which is pivotal in inflammatory processes and immune system regulation, making it ideal for research in immunology, oncology, and related fields. With a rat IgG1 isotype, it guarantees high purity and low endotoxin levels (<1.0 EU/mg), ensuring suitability for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for enhanced stability and efficacy. Enhance your research with this reliable and versatile antibody.
TNF is a cytokine involved in systemic inflammation, being one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be made by many other cell types, such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNF's primary role is in the regulation of immune cells, and it is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication.
| Product Name: | Anti-Mouse TNFα In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0007 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | TN3-19.12 |
| Protein: | Tumor Necrosis Factor-Alpha |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Tumor Necrosis Factor Ligand Superfamily Member 2 (TNFSF2), Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin |
| Isotype: | Armenian Hamster IgG |
| Reactivity: | Mouse |
| Immunogen: | Recombinant mouse TNFα |
| Applications: | IP, N, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | IP, N, WB |
| Recommended Usage: | ELISA Each lot of this antibody is quality control tested by ELISA assay. For use of this antibody as a capture, a concentration range of 2 - 6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse TNF-α recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Armenian Hamster |
| Specificity: | Armenian Hamster Anti-Mouse TNFa (Clone TN3-19.12 ) recognizes Mouse TNFa. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| Antigen Distribution: | Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells |
| Immunogen: | Recombinant mouse TNFα |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity.1 It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites.2 TNF-α is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-α secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-α has been detected in synovial fluid of patients with rheumatoid arthritis. Clone TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-α
| Technical Datasheet: | View |
| Protein: | Tumor Necrosis Factor-Alpha |
| Research Area: | Immunology, Innate Immunity |
| Armenian Hamster IgG Isotype Control | |
|---|---|
| Clone | PIP |
| Isotype | Armenian Hamster IgG |
| Endotoxin Level | Low Endotoxin |
