Description
Anti-Mouse Vγ2 TCR In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse Vβ2 TCR In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the Vβ2 T-cell receptor (TCR), crucial in antigen recognition and immune response, making it ideal for research in immunology and related fields.
With enhanced specificity and ultra-low endotoxin levels (<1.0 EU/mg), it ensures high purity, perfect for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Enhance your research with this reliable and versatile antibody. The Vβ2 TCR is a component of the T-cell receptor complex found on T-cells, playing a significant role in recognizing peptide antigens presented by major histocompatibility complex (MHC) molecules. This interaction is vital for T-cell activation and subsequent immune responses, making the Anti-Mouse Vβ2 TCR antibody a valuable tool for studying T-cell function and dynamics in various experimental contexts.
| Product Name: | Anti-Mouse Vγ2 TCR In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0075 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | UC3-10A6 |
| Protein: | V-gamma2 TCR |
| Product Type: | Monoclonal Antibody |
| Synonyms: | V-gamma2 T-cell receptor, T cell receptor gamma 2 |
| Isotype: | Armenian Hamster IgG |
| Reactivity: | Mouse |
| Immunogen: | G8 mouse T cells |
| Applications: | Depletion, FC, IP |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | Depletion, FC, IP |
| Recommended Usage: | FC It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TCR. A suggested method would be to stain cells expressing TCR with Anti-Mouse TCR at ≤1.0 µg per 1.0 X 106 in a 100 µl total staining volume, followed by Goat. |
| Reactivity: | Mouse |
| Host Species: | Armenian Hamster |
| Specificity: | Armenian Hamster Anti-Mouse V-gamma2 TCR recognizes an epitope on Mouse V-gamma2 TCR. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody was also pathogen tested and third-party certified by IDEXX BioReseach to meet the lowest mycoplasma specification and free of any viral pathogens of concern. |
| Antigen Distribution: | V-gamma 2 T-Cell Receptor (TCR)-bearing T lymphocytes make up a significant proportion of γδ TCR-bearing cells in the late fetal and adult thymus and adult peripheral lymphoid tissues and lung. |
| Immunogen: | G8 mouse T cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules. Clone UC3-10A6 antibody has been shown to deplete γδ T cells when administered in vivo.
| Technical Datasheet: | View |
| Protein: | V-gamma2 TCR |
| Ligand/Receptor: | Antigens |
| Armenian Hamster IgG Isotype Control | |
|---|---|
| Clone | PIP |
| Isotype | Armenian Hamster IgG1 |
| Endotoxin Level | Ultra Low Endotoxin |
