The MSR1 Monoclonal Antibody (CAB2401) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using cutting-edge technology, is highly specific to MSR1 and is validated for use in a variety of applications, including immunohistochemistry and flow cytometry.MSR1, known for its role in the innate immune response, is a key player in the clearance of modified lipoproteins and cell debris, contributing to the maintenance of tissue homeostasis and the regulation of inflammation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MSR1 Monoclonal Antibody
SKU:
CAB2401
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0750
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:6000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cytosol, External Side Of Plasma Membrane, Plasma Membrane.
Calculated MW:
50kDa
Observed MW:
70kDa
This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages.
Purification Method
Affinity purification
Gene ID
4481
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MSR1 Rabbit mAb (CAB2401) at 1:1000 dilution. C6 cells were treated with TPA for 3 hours and LPS for 6 hours of stimulation. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
