The NPHS2 Monoclonal Antibody (CAB2302) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NPHS2 Monoclonal Antibody
SKU:
CAB2302
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC1907
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PDCN, SRN1, NPHS2
Positive Sample:
HeLa, HT-29, 293T
Cellular Localization:
Cell-Cell Junction, Cytoplasmic Side Of Plasma Membrane, Endoplasmic Reticulum, Extracellular Exosome, Plasma Membrane, Slit Diaphragm.
Calculated MW:
42kDa
Observed MW:
35kDa
This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
7827
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NPHS2 Rabbit mAb (CAB2302) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
