The NPHS2 Monoclonal Antibody (CAB2302) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot experiments. By binding to the NPHS2 protein, researchers can detect and analyze its expression in various cell types, making it an ideal choice for studies in nephrology and kidney disease research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NPHS2 Monoclonal Antibody
SKU:
CAB2302
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1907
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PDCN, SRN1, NPHS2
Positive Sample:
HeLa, HT-29, 293T
Cellular Localization:
Cell-Cell Junction, Cytoplasmic Side Of Plasma Membrane, Endoplasmic Reticulum, Extracellular Exosome, Plasma Membrane, Slit Diaphragm.
Calculated MW:
42kDa
Observed MW:
35kDa
This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
7827
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NPHS2 Rabbit mAb (CAB2302) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
