The NTRK1/NTRK2/NTRK3 Antibody (CAB18809) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, raised in rabbits, is highly specific and reactive with human samples. Validated for use in Western blot applications, it targets the NTRK1, NTRK2, and NTRK3 receptors, enabling detection and analysis in a variety of cell types.The NTRK family of proteins, including NTRK1, NTRK2, and NTRK3, play critical roles in neuronal development and function. Dysregulation of these receptors has been implicated in various diseases, including cancer, neurodevelopmental disorders, and chronic pain conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NTRK1/NTRK2/NTRK3 Antibody
SKU:
CAB18809
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
HeLa, HT-29, SH-SY5Y, C6
Cellular Localization:
Axon, Dendrite, Early Endosome, Late Endosome, Plasma Membrane.
Observed MW:
100kDa
Neurotrophic tyrosine kinase (NTRK) is a family of receptor tyrosine kinase.The NTRK gene family contains three members, NTRK1, NTRK2 and NTRK3, which produce TRKA, TRKB and TRKC proteins, respectively.TRK kinases leads to cell differentiation and may play important roles in normal neural functions.Rearrangements in the NTRK genes can result in two genes fusing together and producing altered TRK proteins, which can lead to uncontrolled growth of cancer cells. Neurotrophic tyrosine receptor kinase (NTRK) gene fusions are an actionable biomarker for cancer therapy and can be found in over 25 different types of cancer.
Purification Method
Affinity purification
Gene ID
4914 4915 4916
RRID
AB_2862444
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NTRK1/NTRK2/NTRK3 Rabbit pAb (CAB18809) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Immunofluorescence analysis of C6 cells using NTRK1/NTRK2/NTRK3 Rabbit pAb (CAB18809) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
