The Pan-Succinyl-K Antibody (CAB20503) is a high-quality antibody developed for reliable detection and analysis of target proteins. Succinylation of protein is a kind of post-translational modification of protein mainly occurring on lysine residues. Compared with methylation and acetylation, succinylation of lysine has a greater effect on protein structure and function. Sirt5 is an important de-succinylation modifying enzyme involved in the regulation of a variety of biological processes, such as reactive oxygen species defense, fatty acid metabolism and cell apoptosis
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Pan-Succinyl-K Antibody
SKU:
CAB20503
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
293T, MCF7, C6, Mouse liver, Mouse heart
Observed MW:
10-150 kDa
Succinylation of protein is a kind of post-translational modification of protein mainly occurring on lysine residues. Compared with methylation and acetylation, succinylation of lysine has a greater effect on protein structure and function. Sirt5 is an important de-succinylation modifying enzyme involved in the regulation of a variety of biological processes, such as reactive oxygen species defense, fatty acid metabolism and cell apoptosis
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Pan-Succinyl-K Rabbit pAb (CAB20503) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
