The PAR4 Monoclonal Antibody (CAB4983) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, shows high specificity and reactivity with human samples, making it an ideal choice for experiments such as Western blotting.PAR4 plays a crucial role in mediating platelet response to thrombin, a key mediator of blood coagulation. Dysregulation of PAR4 signaling has been implicated in various cardiovascular diseases, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PAR4 Monoclonal Antibody
SKU:
CAB4983
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1980
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PAR4, Par-4
Positive Sample:
HeLa, SW620, 293T, Mouse lung
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
37kDa
Observed MW:
41kDa
This gene encodes a tumor suppressor protein that selectively induces apoptosis in cancer cells through intracellular and extracellular mechanisms. The intracellular mechanism involves the inhibition of pro-survival pathways and the activation of Fas-mediated apoptosis, while the extracellular mechanism involves the binding of a secreted form of this protein to glucose regulated protein 78 (GRP78) on the cell surface, which leads to activation of the extrinsic apoptotic pathway. This gene is located on the unstable human chromosomal 12q21 region and is often deleted or mutated different tumors. The encoded protein also plays an important role in the progression of age-related diseases.
Purification Method
Affinity purification
Gene ID
5074
RRID
AB_2863403
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PAR4 Rabbit mAb (CAB4983) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using PAR4 Rabbit mAb (CAB4983) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
