The Anti-phospho-c-Jun (T93) Antibody (CABP1218) is a valuable tool for researchers studying the phosphorylation of the c-Jun protein, a key regulator of gene expression and cell proliferation. This polyclonal antibody, raised in rabbits, specifically recognizes the phosphorylated threonine 93 residue of c-Jun and has been validated for use in Western blot applications. By targeting this specific phosphorylation site, researchers can assess the activation status of c-Jun in various cell types and gain insights into its role in signaling pathways related to cell growth, differentiation, and apoptosis.The phosphorylation of c-Jun at threonine 93 is known to be important for the transcriptional activity of the protein and its involvement in cellular responses to stress, growth factors, and cytokines.
Dysregulation of c-Jun phosphorylation has been implicated in various diseases, including cancer, inflammatory disorders, and neurodegenerative conditions. Therefore, understanding the dynamics of c-Jun phosphorylation is crucial for identifying potential therapeutic targets and developing novel treatment strategies for these diseases.Overall, the Anti-phospho-c-Jun (T93) Antibody (CABP1218) offers researchers a reliable tool to investigate the role of c-Jun phosphorylation in cellular signaling pathways and disease pathogenesis. Its high reactivity with human samples and specificity for the phosphorylated form of c-Jun make it a valuable asset for studies in molecular biology, oncology, and immunology.
Product Name:
Phospho-c-Jun-T93 Rabbit Polyclonal Antibody
SKU:
CABP1218
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around T93 of human JUN (NP_002219.1).
Sequence:
TPTP T
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
AP1; p39; AP-1; cJUN; c-Jun; Phospho-c-Jun-T93
Positive Sample:
HeLa,NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
36kDa
Observed MW:
48kDa
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Purification Method:
Affinity purification
Gene ID:
3725
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-c-Jun-T93 Rabbit pAb (CABP1218) at 1:500 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
