Description
Phospho-KIT-Y719 Antibody (CABP0586)
The Phospho-KIT-Y719 Antibody (CABP0586) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, has high specificity for phosphorylated CD117 and is validated for use in techniques such as Western blot and immunohistochemistry.Phosphorylation of the c-Kit protein at the Y719 site is known to be important for its activation and function in various cellular processes, including cell proliferation, differentiation, and survival. By targeting this specific phosphorylation site, researchers can gain insights into the signaling pathways regulated by c-Kit and its role in various physiological and pathological conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | Phospho-KIT-Y719 Antibody |
| SKU: | CABP0586 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | TNEY M | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | PBT, SCFR, C-Kit, CD117, MASTC, Phospho-CD117/c-Kit-Y721 |
| Positive Sample: | NIH/3T3 treated with EGF |
| Cellular Localization: | Cell Membrane, Cytoplasm, Single-Pass Type I Membrane Protein. |
| Calculated MW: | 110kDa |
| Observed MW: | 120kDa/145kDa |
This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
| Purification Method | Affinity purification |
| Gene ID | 3815 |
| RRID | AB_2771250 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CD117/c-Kit-Y721 Rabbit pAb (CABP0586) at 1:500 dilution. NIH/3T3 cells were treated with EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 20μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s. |
