The Anti-Phospho IκBα (S32) Polyclonal Antibody (CABP1220) is a crucial tool for researchers studying phosphorylated IκBα signaling pathways. IκBα is a key player in the regulation of NF-κB, a transcription factor involved in immune and inflammatory responses. The antibody, raised in rabbits, specifically targets phosphorylated IκBα at serine 32, allowing for precise detection and analysis in Western blot applications.Phosphorylation of IκBα at serine 32 is known to trigger its degradation, leading to the activation of NF-κB and subsequent gene transcription.
Understanding the regulation of this process is essential for unraveling the complex mechanisms underlying inflammation, immune response, and various diseases such as cancer, autoimmune disorders, and inflammatory conditions. The Anti-Phospho IκBα (S32) Polyclonal Antibody provides researchers with a reliable tool to investigate and explore the role of phosphorylated IκBα in these pathways, advancing our understanding of immune regulation and disease pathogenesis.
Product Name:
Phospho-IκBα-S32 Rabbit pAb
Product Code:
CABP1220
Size:
50uL, 100uL
Synonyms:
IKBA, MAD-3, NFKBI, IKB alpha, NFKBIA, EDAID2
Applications:
WB
Reactivity:
Human, Mouse
Host Species:
Rabbit
Immunogen:
A phospho specific peptide corresponding to residues surrounding S32 of human NFKBIA.
Applications:
WB
Recommended Dilutions:
WB 1:500 - 1:1000
Reactivity:
Human, Mouse
Positive Samples:
HeLa, NIH/3T3
Immunogen:
A phospho specific peptide corresponding to residues surrounding S32 of human NFKBIA.
Purification Method:
Affinity purification
Storage:
Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:
IgG
Sequence:
Email for sequence
Gene ID:
4792
Uniprot:
P25963
Cellular Location:
Cytoplasm, Nucleus
Calculated MW:
35kDa
Observed MW:
40kDa
UniProt Protein Function:
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
NCBI Summary:
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
