Anti-Phospho-Smad5 (Ser463/465) [R05-2U1] Monoclonal Antibody (AGMB05044) is developed for research use and supports reliable laboratory performance. Suitable for WB, IHC-P, ICC/IF applications, it enables consistent and reproducible experimental results across a wide range of scientific studies. Raised in Rabbit, the antibody demonstrates reactivity with Human, Mouse, Rat samples, ensuring accurate detection within relevant biological systems.
Supplied in Liquid form, it is designed for convenient handling in standard laboratory workflows. For optimal stability and long-term performance, Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles., instructions to preserve antibody activity and ensure dependable research outcomes.
A synthesized peptide derived from human Phospho-Smad5 (S463/465)
Tested Applications:
WBIHC-PICC/IF
Antibody Dilution Ratio:
Application
Antibody Dilution Ratio
WB
1:1000-1:2000
IHC
1:100-1:200
IF
1:50-1:200
Isotype:
IgG
Synonyms:
DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad 5, hSmad5, JV5 1, JV5-1, MAD homolog 5, MAD mothers against decapentaplegic homolog 5, MAD, mothers against decapentaplegic homolog 5, MADH 5, MADH5, Mothers against decapentaplegic homolog 5, Mothers against DPP homolog 5, MusMLP, SMA and MAD related protein 5, SMAD 5, SMAD family member 5, SMAD mothers against DPP homolog 5, Smad5, Smad5, SMAD5_HUMAN.
Clonality:
Monoclonal Antibody
Clone:
R05-2U1
Form:
Liquid
Conjugate:
Unconjugated
Modification:
Phosphorylated
Molecular Weight:
Calculated MW: 52 kDa, Observed MW: 58 kDa
Storage Buffer:
Liquid in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
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