The Rad21 Monoclonal Antibody (CAB19749) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.
This antibody is validated for use in WB, ChIP, ChIP-seq, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Rad21 Monoclonal Antibody
SKU:
CAB19749
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2276
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBChIPChIP-seqELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Condensed Nuclear Chromosome, Cytosol, Nuclear Matrix, Nucleoplasm, Nucleus, Spindle Pole.
Calculated MW:
72kDa
Observed MW:
130kDa
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.
Purification Method
Affinity purification
Gene ID
5885
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Rad21 Rabbit mAb (CAB19749) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Chromatin immunoprecipitation analysis of extracts of A-549 cells, using Rad21 antibody (CAB19749) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Chromatin immunoprecipitation was performed with 10.7 μg of cross-linked chromatin from A-549 cells using 5 μg of Rad21 Rabbit mAb (CAB19749). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of Rad21 in the representative genomic region surrounding MYC gene.
