The SMARCD3 Antibody (CAB20174) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it suitable for use in Western blotting, immunofluorescence, and immunohistochemistry applications.SMARCD3 is known to play a crucial role in gene regulation and chromatin remodeling processes, impacting various cellular functions such as cell proliferation, differentiation, and DNA repair. Dysregulation of SMARCD3 has been linked to cancer development and progression, making it a promising target for cancer research and therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SMARCD3 Antibody
SKU:
CAB20174
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Rsc6p, BAF60C, CRACD3, SMARCD3
Positive Sample:
HeLa, PC-3, Mouse heart, Rat heart
Cellular Localization:
Cytoplasm, Nucleoplasm, Nucleus.
Calculated MW:
55kDa
Observed MW:
58kDa
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Purification Method
Affinity purification
Gene ID
6604
RRID
AB_2862961
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SMARCD3 Rabbit pAb (CAB20174) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
