The SMC4 Monoclonal Antibody (CAB3559) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene belongs to the 'structural maintenance of chromosomes' (SMC) gene family. Members of this gene family play a role in two changes in chromosome structure during mitotic segregation of chromosomes- chromosome condensation and sister chromatid cohesion. The protein encoded by this gene is likely a subunit of the 13S condensin complex, which is involved in chromosome condensation. A pseudogene related to this gene is located on chromosome 2. RRID AB_2863086 Gene ID 10051 Swiss Prot Synonym CAPC; CAP-C; SMC-4; SMC4L1; SMC4
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SMC4 Monoclonal Antibody
SKU:
CAB3559
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2042
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CAPC, CAP-C, SMC-4, SMC4L1, SMC4
Positive Sample:
HT-29, NIH/3T3, Raji, C6, Mouse testis, Mouse thymus, Rat testis
Cellular Localization:
Chromosome, Cytoplasm, Nucleus.
Calculated MW:
147kDa
Observed MW:
180kDa
This gene belongs to the 'structural maintenance of chromosomes' (SMC) gene family. Members of this gene family play a role in two changes in chromosome structure during mitotic segregation of chromosomes- chromosome condensation and sister chromatid cohesion. The protein encoded by this gene is likely a subunit of the 13S condensin complex, which is involved in chromosome condensation. A pseudogene related to this gene is located on chromosome 2. RRID AB_2863086 Gene ID 10051 Swiss Prot Synonym CAPC; CAP-C; SMC-4; SMC4L1; SMC4
Purification Method:
Affinity purification
Gene ID:
10051
RRID:
AB_2863086
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SMC4 Rabbit mAb (CAB3559) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of C6 cells using SMC4 Rabbit mAb (CAB3559) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using SMC4 Rabbit mAb (CAB3559) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
