The TARBP1 Antibody (CAB20524) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using monoclonal technology, shows high specificity and sensitivity for detecting TARBP1 in human samples, making it suitable for use in applications such as Western blotting and immunofluorescence.TARBP1, also known as Trans-activation response RNA-binding protein 1, is essential for the processing of microRNA molecules and plays a critical role in gene regulation and cell function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
TARBP1 Antibody
SKU:
CAB20524
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TRM3, TRMT3, TRP185, TRP-185, TARBP1
Positive Sample:
293T, U2OS
Cellular Localization:
Nucleus.
Calculated MW:
182kDa
Observed MW:
182kDa
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. This element forms a stable stem-loop structure and can be bound by either the protein encoded by this gene or by RNA polymerase II. This protein may act to disengage RNA polymerase II from TAR during transcriptional elongation. Alternatively spliced transcripts of this gene may exist, but their full-length natures have not been determined.
Purification Method
Affinity purification
Gene ID
6894
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TARBP1 Rabbit pAb (CAB20524) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
