The TCF4/TCF7L2 Polyclonal Antibody (CAB20770) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for human samples and is validated for use in Western blot applications. By binding to TCF4 and TCF7L2 proteins, this antibody enables researchers to detect and analyze these transcription factors in various cell types, making it an essential reagent for studies in molecular biology and developmental biology.TCF4 and TCF7L2 are key players in the Wnt signaling pathway, which is critical for embryonic development, tissue homeostasis, and cancer progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
TCF4/TCF7L2 Polyclonal Antibody
SKU:
CAB20770
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
TART LHFQ SGST HYSA YKTI EHQI AVQY LQMK WPLL DVQA GSLQ SRQA LKDA RSPS PAHI VSNK V
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TCF4, TCF-4, TCF4/TCF7L2
Positive Sample:
SW620, Mouse brain
Cellular Localization:
Nucleoplasm, Nucleus, Pml Body.
Calculated MW:
68kDa
Observed MW:
60kDa/75kDa
This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
6934
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TCF4/TCF7L2 Rabbit pAb (CAB20770) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
