The TIN2/TINF2 Monoclonal Antibody (CAB9750) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes one of the proteins of the shelterin, or telosome, complex which protects telomeres by allowing the cell to distinguish between telomeres and regions of DNA damage. The protein encoded by this gene is a critical part of shelterin; it interacts with the three DNA-binding proteins of the shelterin complex, and it is important for assembly of the complex. Mutations in this gene cause dyskeratosis congenita (DKC), an inherited bone marrow failure syndrome.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TIN2/TINF2 Monoclonal Antibody
SKU:
CAB9750
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1732
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TIN2, DKCA3, TIN2/TINF2
Positive Sample:
Hep G2, A549, Mouse testis, Mouse thymus, Rat lung, Rat testis
Cellular Localization:
Chromosome, Nucleus, Nucleus Matrix, Telomere.
Calculated MW:
50 kDa
Observed MW:
45 kDa
This gene encodes one of the proteins of the shelterin, or telosome, complex which protects telomeres by allowing the cell to distinguish between telomeres and regions of DNA damage. The protein encoded by this gene is a critical part of shelterin; it interacts with the three DNA-binding proteins of the shelterin complex, and it is important for assembly of the complex. Mutations in this gene cause dyskeratosis congenita (DKC), an inherited bone marrow failure syndrome.
Purification Method
Affinity purification
Gene ID
26277
RRID
AB_2863771
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TIN2/TINF2 Rabbit mAb (CAB9750) at 1:1000 dilution incubated overnight at 4°C Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
