The TOMM22 Monoclonal Antibody (CAB9666) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is an integral membrane protein of the mitochondrial outer membrane. The encoded protein interacts with TOMM20 and TOMM40, and forms a complex with several other proteins to import cytosolic preproteins into the mitochondrion.
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TOMM22 Monoclonal Antibody
SKU:
CAB9666
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1688
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:100 - 1:1000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
1C9-2, TOM22, MST065, MSTP065, TOMM22
Positive Sample:
HeLa, Hep G2, K-562, Mouse brain, Mouse spleen, Rat lung, Rat brain
The protein encoded by this gene is an integral membrane protein of the mitochondrial outer membrane. The encoded protein interacts with TOMM20 and TOMM40, and forms a complex with several other proteins to import cytosolic preproteins into the mitochondrion.
Purification Method
Affinity purification
Gene ID
56993
RRID
AB_2863754
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using TOMM22 Rabbit mAb (CAB9666) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using TOMM22 Rabbit mAb (CAB9666) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Immunoprecipitation analysis of 300 μg extracts of K-562 cells using 3 μg TOMM22 antibody (CAB9666). Western blot was performed from the immunoprecipitate using TOMM22 antibody (CAB9666) at a dilution of 1:1000.
