The TRUB1 Antibody (CAB20492) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity towards human samples and is validated for use in Western blot applications. By specifically binding to the TRUB1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an excellent choice for investigations in molecular biology and RNA biology.TRUB1, also known as tRNA pseudouridine synthase B, plays a crucial role in catalyzing the formation of pseudouridine in tRNA molecules, which is essential for proper tRNA function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TRUB1 Antibody
SKU:
CAB20492
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PUS4, TRUB1
Positive Sample:
HeLa, A-549, RD, Mouse liver, Mouse skeletal muscle, Rat liver, Rat brain
Cellular Localization:
Cytosol, Mitochondrion, Nucleus.
Calculated MW:
37kDa
Observed MW:
37kDa
Pseudouridine is an abundant component of rRNAs and tRNAs and is enzymatically generated by isomerization of uridine by pseudouridine synthase (Zucchini et al., 2003 [PubMed 12736709]).
Purification Method
Affinity purification
Gene ID
142940
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TRUB1 Rabbit pAb (CAB20492) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
