The TWEAKR/Fn14/CD266 Monoclonal Antibody (CAB5955) is a high-quality antibody developed for reliable detection and analysis of target proteins. Involved in positive regulation of extrinsic apoptotic signaling pathway and regulation of wound healing. Predicted to be located in cell surface and ruffle. Predicted to be active in plasma membrane.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
TWEAKR/Fn14/CD266 Monoclonal Antibody
SKU:
CAB5955
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC2119
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:4000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FN14, CD266, TWEAKR, TWEAKR/Fn14/CD266
Positive Sample:
ACHN, C2C12
Cellular Localization:
Plasma Membrane.
Calculated MW:
14kDa
Observed MW:
14-17kDa
Involved in positive regulation of extrinsic apoptotic signaling pathway and regulation of wound healing. Predicted to be located in cell surface and ruffle. Predicted to be active in plasma membrane.
Purification Method
Affinity purification
Gene ID
51330
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TWEAKR/Fn14/CD266 Rabbit mAb (CAB5955) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
