The WRAP53 Antibody (CAB20167) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody is generated in rabbits and exhibits high reactivity with human samples, making it ideal for use in immunofluorescence and immunoprecipitation techniques. By binding to WRAP53, the antibody allows for the detection and analysis of this protein in a variety of cell types, supporting studies in DNA repair, telomere biology, and cancer research.WRAP53, also known as WD40-encoding RNA antisense to p53, functions as a scaffold protein that helps recruit factors involved in DNA repair pathways to sites of DNA damage.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
WRAP53 Antibody
SKU:
CAB20167
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DKCB3, TCAB1, WDR79, WRAP53
Positive Sample:
Mouse kidney
Cellular Localization:
Cajal Body, Cytosol, Nuclear Body, Nucleoplasm.
Calculated MW:
59kDa
Observed MW:
72kDa
This gene encodes an essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex required for telomere synthesis. This protein is enriched in Cajal bodies, nuclear sites of RNP processing that are important for telomerase function. It interacts with dyskerin, TERT and TERC, other components of active telomerase, and with small Cajal body RNAs (scaRNAs), which are involved in modifying splicing RNAs. This mRNA also functions as a p53 antisense transcript, that regulates endogenous p53 mRNA levels and further induction of p53 protein by targeting the 5' untranslated region of p53 mRNA. Alternatively spliced transcript variants which differ only in the 5' UTR have been found for this gene.
Purification Method
Affinity purification
Gene ID
55135
RRID
AB_2862954
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse kidney, using WRAP53 Rabbit pAb (CAB20167) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
