The AP2A2 Antibody (CAB5391) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and is highly specific for human samples, making it an ideal choice for Western blot experiments.AP2A2 is a key player in the process of internalizing cell surface receptors and other molecules, regulating important cellular functions such as signaling and nutrient uptake. By targeting the AP2A2 protein, researchers can gain insights into the mechanisms of endocytosis and its role in various physiological processes.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
AP2A2 Antibody
SKU:
CAB5391
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane.
Purification Method
Affinity purification
Gene ID
161
RRID
AB_2766200
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Alpha2-adaptin/AP2A2 Rabbit pAb (CAB5391) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from SH-SY5Y cells, using Alpha2-adaptin/AP2A2 Rabbit pAb (CAB5391) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using Alpha2-adaptin/AP2A2 Rabbit pAb (CAB5391) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
