The AP3S2 Antibody (CAB17607) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the AP3S2 protein, this antibody enables precise detection and analysis in various cell types, making it an essential component for investigations in cell biology and molecular biology research.AP3S2 plays a crucial role in vesicular trafficking processes, ensuring the proper delivery of cargo molecules to their designated destinations within the cell.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
AP3S2 Antibody
SKU:
CAB17607
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AP3S3, sigma3b, AP3S2
Positive Sample:
HT-29, Mouse brain, Rat brain
Cellular Localization:
Axon Cytoplasm, Early Endosome, Golgi Apparatus, Presynapse.
Calculated MW:
22kDa
Observed MW:
22kDa
Predicted to be involved in anterograde synaptic vesicle transport and vesicle-mediated transport. Located in intracellular membrane-bounded organelle. Part of AP-3 adaptor complex.
Purification Method
Affinity purification
Gene ID
10239
RRID
AB_2768386
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using AP3S2 Rabbit pAb (CAB17607) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of C6 cells using AP3S2 Rabbit pAb (CAB17607) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
