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APC Anti-Mouse CD34 Antibody [RAM34] (AGEL3006)

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SKU AGEL3006
Product Type Antibody
Antibody Type Monoclonal Antibody
Applications FCM
Reactivity Mouse
Host Species Rat
Isotype Rat IgG2a, κ
Clone RAM34
Conjugation APC
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Description

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APC Anti-Mouse CD34 Antibody [RAM34]

The APC Anti-Mouse CD34 Antibody (RAM34) is a vital tool for researchers studying the CD34 cell surface marker, an important factor in hematopoietic stem cell identification and isolation. This antibody, specifically designed for use in flow cytometry applications, is highly specific for mouse samples and has been rigorously validated for reliable and accurate results. By targeting the CD34 protein, this antibody enables precise detection and analysis of CD34-expressing cells in various tissues and cell populations, making it ideal for research in stem cell biology, hematopoiesis, and vascular biology.CD34 is a transmembrane glycoprotein that is widely used as a marker for identifying hematopoietic stem and progenitor cells, as well as endothelial progenitor cells.

Its expression pattern and function play crucial roles in processes such as hematopoiesis, angiogenesis, and tissue regeneration. Researchers studying stem cell differentiation, transplantation, and regenerative medicine rely on accurate detection of CD34 expression to understand and manipulate these processes effectively.By utilizing the APC Anti-Mouse CD34 Antibody (RAM34), researchers can confidently analyze and characterize CD34-positive cell populations with precision and sensitivity. This antibody provides a powerful tool for advancing scientific discoveries in stem cell research, development of cell-based therapies, and understanding the intricate mechanisms underlying hematopoiesis and vascular development.

Product Title:APC Anti-Mouse CD34 Antibody [RAM34]
SKU:AGEL3006
Size:50 Tests, 100 Tests, 200 Tests
Clone No:RAM34
Synonyms:Hematopoietic progenitor cell antigen CD34;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG2a, κ
Isotype Control:APC Rat IgG2a, κ Isotype Control[2A3]
Form:Liquid
Conjugation:APC
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:Spectrum Image
Swissprot:Q64314
Gene ID:12490
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:The RAM34 antibody reacts with the CD34 glycoprotein on the surface of three independently derived mouse CD34-transfected cell lines. RAM34 antibody also reacts with the mouse cell lines PA6, 416B, Swiss 3T6, NIH, 3T3, DA1, and M1, all of which are positive for expression of mouse CD34 mRNA. Cell lines shown to be negative for CD34 transcript, including WEHI-3B, EL4, 18.8, and CMT64/61, are also negative for surface expression of CD34 as determined by RAM34 staining. Normal thymocytes and splenocytes are negative for CD34 expression. In the bone marrow, 7-10% of cells are stained with RAM34 mAb, including most of the Ly-6A/E (Sca-1)+ CD90 (Thy-1)low Lineage Marker- hematopoietic stem cell-enriched subpopulation and myeloerythroid progenitors. CD34 is also expressed on a small percentage of fetal liver cells, including NK-cell progenitors. CD34 has been reported to be expressed on the endothelium of capillaries and, in this form, to function as a ligand for L-selectin. Consistent with this observation, RAM34 antibody stains endothelial cells in spleen, thymus, and postcapillary HEVs in the lymph nodes. It is reported that RAM34 antibody can be used to select CD34+ CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker- bone marrow-derived hematopoietic stem cells, capable of short-term multi-lineage reconstitution of lethally irradiated mice; while the CD34- CD117+ Sca-1+ Lineage Marker- population contains self-renewing hematopoietic stem cells. Similarly, the bone marrow population with high dye-efflux capacity and which is highly enriched for long-term reconstituting hematopoietic stem cells is CD34- CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker-.

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