The APC Monoclonal Antibody (CAB17912) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Mutations in the APC gene have been found to occur in most colorectal cancers, where disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. RRID AB_2861757 Gene ID 324 Swiss Prot Synonym GS; DP2; DP3; BTPS2; DESMD; DP2.5; PPP1R46; APC
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
APC Monoclonal Antibody
SKU:
CAB17912
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0346
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:1000 - 1:2000
IP
0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Mutations in the APC gene have been found to occur in most colorectal cancers, where disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. RRID AB_2861757 Gene ID 324 Swiss Prot Synonym GS; DP2; DP3; BTPS2; DESMD; DP2.5; PPP1R46; APC
Purification Method:
Affinity purification
Gene ID:
324
RRID:
AB_2861757
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using APC Rabbit mAb (CAB17912) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Immunoprecipitation analysis of 600 μg extracts of Mouse lung using 3 μg APC antibody (CAB17912). Western blot was performed from the immunoprecipitate using APC antibody (CAB17912) at a dilution of 1:500.
