The ARFRP1 Antibody (CAB14311) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity in detecting ARFRP1 in human samples, making it an ideal choice for Western blotting experiments.ARFRP1 plays a crucial role in regulating cellular processes such as endocytic recycling and protein transport within the cell. Its dysregulation has been linked to various diseases, including cancer and neurodegenerative disorders. By targeting ARFRP1 and studying its function, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ARFRP1 Antibody
SKU:
CAB14311
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ARP, Arp1, ARL18, ARFRP1
Positive Sample:
U-87MG, 293T, Mouse brain, Mouse heart, Rat lung
Cellular Localization:
Golgi Apparatus, Trans-Golgi Network.
Calculated MW:
23kDa
Observed MW:
25kDa
The protein encoded by this gene is a membrane-associated GTP-ase which localizes to the plasma membrane and is related to the ADP-ribosylation factor (ARF) and ARF-like (ARL) proteins. This gene plays a role in membrane trafficking between the trans-Golgi network and endosomes. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method
Affinity purification
Gene ID
10139
RRID
AB_2761175
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ARFRP1 Rabbit pAb (CAB14311) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
