The ARHGAP1 Antibody (CAB3739) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ARHGAP1 Antibody
SKU:
CAB3739
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RHOGAP, RHOGAP1, CDC42GAP, p50rhoGAP, ARHGAP1
Positive Sample:
BxPC-3, HeLa, HepG2, U-87MG, PC-12, Mouse lung
Cellular Localization:
Cytoplasm.
Calculated MW:
50kDa
Observed MW:
50kDa
This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members.
Purification Method
Affinity purification
Gene ID
392
RRID
AB_2765241
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ARHGAP1 Rabbit pAb (CAB3739) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg ARHGAP1 antibody (CAB3739). Western blot was performed from the immunoprecipitate using ARHGAP1 antibody (CAB3739) at a dilution of 1:1000.
