The ARHGAP9 Monoclonal Antibody (PAC022569) is a valuable tool for researchers studying ARHGAP9, a key protein involved in cell signaling and cytoskeleton organization. This antibody, generated in a mouse host, is highly specific for human samples and has been rigorously validated for use in applications such as immunofluorescence and flow cytometry. By targeting the ARHGAP9 protein, this antibody facilitates the visualization and analysis of ARHGAP9 expression in various cell types, offering valuable insights for studies in cell biology and cancer research.
ARHGAP9, a member of the RhoGAP protein family, plays a crucial role in regulating Rho GTPase activity, which in turn influences processes such as cell migration, adhesion, and proliferation. Dysregulation of ARHGAP9 has been implicated in cancer progression and metastasis, making it a promising target for therapeutic intervention. By investigating the function of ARHGAP9, researchers can gain a better understanding of its role in disease pathogenesis and potentially identify new opportunities for targeted therapy development.
Antibody Name:
ARHGAP9 Antibody (PACO22569)
Antibody SKU:
PACO22569
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human RHG9.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from LOVO cells, using RHG9 antibody.
Background:
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has a substantial GAP activity toward CDC42 and RAC1 and less toward RHOA. Has a role in regulating adhesion of hematopoietic cells to the extracellular matrix. Binds phosphoinositides, and has the highest affinity for phosphatidylinositol 3,4,5-trisphosphate, followed by phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 4,5-bisphosphate.
Synonyms:
RGL1; RHG09; rho GTPase activating 9;
UniProt Protein Function:
ARHGAP9: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has a substantial GAP activity toward CDC42 and RAC1 and less toward RHOA. Has a role in regulating adhesion of hematopoietic cells to the extracellular matrix. 4 isoforms of the human protein are produced by alternative splicing.Protein type: GAPs; Cell adhesion; GAPs, Rac/RhoChromosomal Location of Human Ortholog: 12q13.3Cellular Component: cytosolMolecular Function: phosphatidylinositol-3,4,5-triphosphate binding; GTPase activator activityBiological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the Rho-GAP family of GTPase activating proteins. The protein has substantial GAP activity towards several Rho-family GTPases in vitro, converting them to an inactive GDP-bound state. It is implicated in regulating adhesion of hematopoietic cells to the extracellular matrix. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
